![]() ![]() Users can find photos in the imported folder according to: It will either be Single List Mode or Side to Side Mode.Īfter dragging and dropping the folder to PhotoSweeper, all the photos will be displayed on the interface, but how to find duplicates fast? Under the “Find Photos”, PhotoSweeper allows users to access to different settings to find the duplicates fast and easily. That’s to say, you can drag and drop a folder or image into each side of this window, the program will find and compare duplicates storing in these 2 folders or images.īy choosing this mode, the program will restore photos and comparison results from the previous session. It allows users to find duplicates between two sets of photos. It allows users to find duplicates in one set of photos, that is to say, you can drag and drop a folder to the program, then the program will scan and compare duplicates within this single folder. On the Welcome screen, you will be guided to choose from 3 modes: Single List Mode, Side to Side Mode, Restore Last Session. ![]() The interface will depend on which mode you choose and the main toolbars are located on the bottom of each interface. Users can directly update in the Mac App Store. For example, in the latest update (version 3.7.0) as of May 2020, it added support for more video formats such as AVI, which means users are now able to use it to remove duplicate AVI files. PhotoSweeper has been updated to add new support and improve user experience. Therefore, for users who carry out the 1st installation, there will be an individual window with several slides prompting necessary PhotoSweeper tips on how to use this tool. PhotoSweeper allows users to access to its abundant settings to remove duplicates, which make this program a little complicate than others. To tell the truth, PhotoSweeper works good to find and remove duplicate photos, but it is not really simple to use, especially when you want to utilize some of its features for accurate scanning and removel. Users can download the program on its official website, the installation package is 28MB and will take a space of 34 MB on your Mac for the installation. Provide 3 ways to remove unwanted selected duplicates.Select duplicates automatically for batch removal in 1 click.Offer 3 preview modes, allowing side-by-side preview. ![]() Quickly scan for duplicates and let you exclude folders from scan.Remove duplicates from Mac’s Photos, iPhoto, iTunes and Music.Support computer’s internal HD, external HD, SD card, USB flash drive and more.Find duplicate images, audios, videos, documents, packages, etc.Cisdem Duplicate Finder The Best PhotoSweeper Alternative ![]()
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![]() ![]() Most home loans require a down payment of at least 3%. The amount of money you spend upfront to purchase a home. ![]() For example, if you have a $250 monthly car payment and $50 minimum credit card payment, your monthly debt would be $300. You can adjust this amount in our affordability calculator as needed. These are recurring monthly expenses like car payments, minimum credit card payments or student loans. If you have a co-borrower who will contribute to the mortgage, combine the total of both incomes to get your annual income. You can usually find the amount on your W2 form. This is the total amount of money earned for the year before taxes and other deductions. Learn more about the line items in our calculator to determine your ideal housing budget. Our calculator also includes advanced filters to help you get a more accurate estimate of your house affordability, including specific amounts of property taxes, homeowner's insurance and HOA dues (if applicable). ![]() You can calculate affordability based on your annual income, monthly debts and down payment, or based on your estimated monthly payments and down payment amount. An interest-only period is a term during which all payments are counted against interest on the loan.Zillow's affordability calculator allows you to customize your payment details, while also providing helpful suggestions in each field to get you started. A balloon payment represents a balance due at the end of the loan term. Points (also called discount points or mortgage points) are a way for borrowers to reduce the overall interest rate by making an up-front payment one point equals one percent of the loan amount. Mortgage computations can also include additional features and variables. By adjusting loan amounts and interest rates as well as different down payment amounts, you can determine the size of mortgage you might be able to afford and calculate recurring costs over time. From these variables, Wolfram|Alpha can compute monthly payments, total interest charges and breakdowns of payments against the principal loan amount and interest over the life of the mortgage. The simplest mortgage calculation requires only a total loan amount, a loan period and the annual percentage rate of interest on the loan. Several different types (5/1, 1/1, 3/1, 7/1) of adjustable-rate mortgages and rate cap structures (5/2/5, 2/2/6, 2/2/5) are available to choose from. In a fixed-rate mortgage, the interest rate remains the same over the life of the loan in an adjustable-rate mortgage, the interest rate is typically fixed for some period of time, but then may fluctuate in response to changing market indices that influence interest rates. Understanding mortgage calculations Wolfram|Alpha can perform useful computations related to common mortgage types. ![]() Partial Fraction Decomposition Calculator.home loan 100,000 at 3.7 percent apr for 30 years $10,000 balloon payment 1% points.$200k adjustable mortgage with 20% down payment.Your input can include complete details about loan amounts, down payments and other variables, or you can add, remove and modify values and parameters using a simple form interface. You can calculate payoff times and total interest payments generate plots and monthly payment tables showing balances over time and dynamically compute the effects of different down payments, interest rates and mortgage types.Įnter your queries using plain English. Wolfram|Alpha is an excellent tool for calculating the total cost, monthly payments and other costs and times associated with common mortgage types. Assuming loan amount | Use sale amount and down payment instead Simple yet powerful mortgage research and computation ![]() ![]() ![]() The primary aim of a 3D ultrasound pregnancy check-up is to ensure the fetus’ health, which includes measuring physical activity and fetal heart rate, as well as the placenta, amniotic fluid, and fetal external organs. Why Are Pregnancy Examinations with a 3D Ultrasound Necessary? Choose a reputable hospital or other healthcare facility to ensure its protection to undergo a 3D ultrasound examination. Pregnant women, on the other hand, should have a 3D ultrasound examination according to the obstetrician’s schedule or prescription. By using the equipment and products used, pregnant women and their fetuses are totally healthy. To date, there have been no reports about the risks of a 3D ultrasound prenatal examination. In the early stages of pregnancy, a 2D ultrasound may be used to perform the test since the form of the fetus is not apparent and the size is still small at the beginning of pregnancy, resulting in a poor 3D image. The best time for a 3D ultrasound test is between 26 to 30 weeks of pregnancy. Who Needs a 3D Ultrasound Examination and When Is the Best Time to Get One?Ī 3D ultrasound pregnancy check-up is available to all pregnant women. As a result, a 3D ultrasound scan will identify any anomalies in the fetus, allowing doctors to take prompt action. An obgyn doctor may also obtain more detailed details about the fetus’ organs, such as the heart.ģD ultrasound pregnancy examination results images, in comparison to two-dimensional ultrasound, display a fuller fetal scale, including height, width, and volume. The obstetrician will also examine the fetus in greater detail in order to identify abnormalities. ![]() The three-dimensional images generated by a 3D ultrasound pregnancy analysis are more visible. A three-dimensional (3D) ultrasound pregnancy test is a medical technique that uses ultrasound to assess the state of the fetus in three dimensions. ![]() ![]() You can also achieve this on a charcoal grill by positioning the hot coals on one side and leaving the other side empty. ![]() Instead, you can spice it up with fresh herbs, spices, and garlic. Lamb is already a tender cut, so theres no need to treat it with acidic ingredients. On the flip side, the Kitchn suggests skipping the marinade altogether. (See infographic below for a visual of what I mean.) You'll start the lamb loin chops on the cool side and then finish them on the hot side for those beautiful grill marks. They go on to say even 15 to 20 minutes might be enough, especially for thinner cuts like lamb chops. In this recipe, you'll use a simple technique called two-zone grilling, which just means you create a 'cool zone' and a 'hot zone' on your grill. The moment they hit they the grill and start to sizzle, you know they'll be magnificent. But the easy blend of fresh rosemary and garlic swirled with good olive oil, salt and pepper work magic to enhance lamb's distinct flavor and succulent texture. When you read the ingredient list, it may seem too simple. Black Pepper: freshly ground black pepper.Garlic: look for a firm, plump head of garlic with smooth skin Starting the lamb chops on the cool side of a two-zone grill fire cooks them through evenly without flare-ups or too much char.Rosemary: fresh rosemary, dried rosemary isn't a good substitute here.Olive Oil: good, extra-virgin olive oil.Lamb Loin Chops: about 1 ½-inches thick, to use lamb rib chops, use this Grilled Lamb Chops recipe instead.Ingredients You Need to Make Grilled Lamb Loin Chops ![]() A little bit of forethought means a mostly hands-off main course. It takes time for the marinade to permeate the thick cut chops, so plan to prepare them at least an hour in advance or overnight for extra flavor. Add the lamb and grill until deep golden brown on all sides, including the fatty edges, and the internal temperature is 125✯ for medium doneness or 145✯ for medium-well doneness. ![]() When you want an impressive meal that you can put together in 10 minutes, look no further than these succulent grilled lamb loin chops. Heat a cast iron grill pan over medium-high heat. Weeknight easy and splendid on a platter for dinner parties and date nights. This allows the juice to redistribute adding to the flavor and the surface heat of the meat will distribute more evenly to the center.Slathered in a rich, aromatic blend of olive oil, fresh rosemary and garlic, Grilled Lamb Loin Chops come out juicy and perfect every time. Allow grilled cuts to rest for 5-8 minutes before serving.(Internal temperature 125-130 F for rare to medium-rare) You may need to take the meat off the grill momentarily to do so. On chops or steaks push the probe 2-3” into the side of the cut for more accurate readings. Take the temperature at the center of the cut of meat, not next to a bone. A meat thermometer is a more accurate gauge of being done than color. 03 of 11 Grilled Garlic and Rosemary Lamb Loin Chops View Recipe Soup Loving Nicole These grilled chops marinated in lemon juice, garlic, rosemary, and Dijon mustard pair perfectly with a garlic-rosemary aioli, according to recipe creator seren.Use tongs to turn, not a fork, to avoid piercing the meat surface allowing the juice to escaping.After searing use moderate heat to finish cooking. If it begins to char or flares up move the cut to a less intense area of the grill. ![]() Sear the meat on each side until deeply browned but not charred.Then adjust the heat so that one area of the grill is at full heat and another at moderate heat. Flip the chops over and cook for 1 minute before adding a knob of butter with the rosemary and garlic. Knock the chops onto their sides and cook for 2 minutes. If not much fat has rendered out and the pan is still quite dry, add a small dash of oil. On a charcoal or gas grill preheat all sections. Render down the fat for a few minutes until golden and crisp.The meat will grill quicker and more consistently. Allow the meat cuts to sit out at room temperature before grilling rather than cooking straight from the refrigerator.Thaw frozen cuts slowly in the refrigerator or place the vacuum sealed package in cold water. Quickening the process in a microwave is likely to lessen the quality of the meat. Grill the chops for 2 to 3 minutes per side. Reduce the heat down to a medium and close the grill lid. You want the grill grates to be extra, extra hot Place the garlic lamb chops about 3 to 4 inches apart on the grill. Remember to thaw your meat cuts ahead of time. When you’re ready to grill the chops, preheat an outdoor grill to 500F for 5 minutes. ![]() ![]() ![]() Choosing us means getting decades of experience in guiding potential pool table owners in the right direction. Here's why we believe we can be that pool table supply store for you. Sometimes finding the perfect pool table for you comes down to finding the perfect pool table supply store. There are a lot of different designs available when it comes to our table selection, and the best one for you is going to depend on nothing more than your personal sensibilities. Style is always subjective, and that's no different when it comes to pool tables. Any less than that and you may wind up slamming your cue into a wall when you're going to shoot. If you don't have room for a nine-foot table in your home, you can easily eliminate a number of available options.Īs a general rule for pool table size, you want to make sure that you leave at least five feet of open space around the perimeter of your table in the room it's intended for. Size is one of the simplest but most important factors when choosing a pool table. Then, you can start sifting through the options in your price range. Figure out what you're looking to spend first. ![]() However, there are both advantages and potential disadvantages to each and every one of them. Billiards tables come in a range of prices, and there is a significant difference in cost between the lower end and the top of the top. The easiest way to narrow down your search is to determine a budget. For instance, you should consider the following: Budget Finding the Right Pool Tableįinding the perfect billiards tables for you and your home is going to depend on a few different factors. If you want to know more about the brands and models we carry, reach out to us today or stop by the nearest location. Some of the most popular brands we offer include: We're confident you'll be able to find something that matches both your budget and sensibilities. Our Pool Tablesīilliard Factory offers a wide array of superior-quality pool tables in a range of prices and styles. And every member of our team has the knowledge and experience necessary to fill you in on all you need to know before you bring your pool table home. ![]() We only carry the most reputable, high-quality brands. ![]() ![]() ![]() ![]() NEW: Pesticides External website, opens in new tabĪccepted Plastic containers (jars, jugs, bottles)Īccepted Printed paper and paper packaging (newspaper, magazines, catalogues, writing paper, paper bags)Īccepted Propane tanks (maximum 4 disposable and 2 refillable)Īccepted Smoke and carbon monoxide alarmsĪccepted Tires (passenger or light truck with or without rims, maximum 10)Īccepted Used oil (maximum 15 L), oil filters (maximum 3), and empty oil containers NEW: Paint and empty paint cans External website, opens in new tabĪccepted Paper containers (tetra-packs, coffee cups) ![]() NEW: Flammable liquids External website, opens in new tabĪccepted Foam packaging (Styrofoam) - no foam peanutsĪccepted Large appliances (refrigerators, freezers, air conditioners)Īccepted Lawnmowers - electric, battery, or gas-powered (drain gasoline and oil before recycling)Īccepted Lead acid car and truck batteriesĪccepted Metal containers (cans, tins, foil, empty aerosol cans excluding spray paint cans)Īccepted Other flexible plastic packaging External website, opens in new tab NEW: Aerosol paint and empty containers External website, opens in new tabĪccepted Antifreeze and empty antifreeze containersĪccepted Clothing and textiles, including reusable bags (items must be clean and dry)Īccepted Cooking oil and grease (maximum 10 L per day)Īccepted Electronics, small appliances, and power tools (maximum 5 large items) Important information Recycling drop-off at landfill is for residential customers only. 8417.Īccepted Materials accepted for free recycling The complete tipping fee details are found in Solid Waste By-law No. Declaration and acknowledgement form required, see drywall policy.Transaction fee: $5 / load (included in minimum fee).Recycling mattresses with bedbugs or more than 8 pieces.Includes mattresses of all types (foam, futon, box springs).Sod and soil, and plastic bags (even those labelled "biodegradable" or "compostable") are not accepted as yard trimmings Includes grass, leaves, and branches (up to 45cm diameter and 2.6m long).Includes solid wood, lumber, or pallets that do not contain glue and have not been painted, stained, or treated.Wood can be painted, stained or treated (other than with creosote).Includes solid wood, lumber, pallets and wood with glue (plywood, particle board, oriented strand board, laminated wood).Soil from businesses and contractors is not accepted for disposal.Ĭonstruction and demolition processing residual waste Minimum volume: 50 m3 (tandem or tridem trailer).Used drywall with asbestos is not accepted from businesses or contractors.Ĭonstruction and demolition waste (C and D) ![]()
![]() Again, this will either be single-bureau or triple-bureau, and we always prefer the latter. In a similar vein, most identity protection services worth their salt will also offer you access to your credit report. By monitoring all three bureaus, you’ll have the best chance of catching unusual activity quickly and intervening before it becomes a major problem. While some might think that’s overkill, the fact of the matter is the three credit bureaus determine scores based on disparate data sets using slightly different algorithms. We always recommend choosing a service or plan that offers triple-bureau monitoring. ![]() A sharp, unexpected decline in your credit score can be a red flag that someone has taken out a loan or opened a new line of credit in your name and skipped out on the bill. Pro Tip: Monitoring your credit score is a good indication of whether or not your identity has been compromised. Credit monitoring will either be single-bureau, meaning the plan only monitors the report from one bureau, or triple-bureau, meaning you’ll have all three bureaus monitored simultaneously. You probably know them as Experian, TransUnion, and Equifax. There are three main credit reporting agencies, also called bureaus, that keep track of your credit score. Keep in mind that any identity protection plan that doesn’t offer both probably isn’t worth your time, but all identity theft protection services that offer these core functions do so in various ways.įirst, let’s talk about credit monitoring. We consider core features to be twofold: credit monitoring and identity protection. There are tons of identity theft protection services on the market, and for the most part, they all offer core features with some unique bonuses, depending on which one you go with. Core Features to Look for When Comparing ID Theft Protection ![]() Now let’s have a look at the most important features you’ll want to look for in any identity theft protection plan. Don’t forget to cancel before the trial period ends, otherwise you’ll continue to get charged. Just be careful to stay on top of it, though. Once you find a service you like, it should be pretty easy to find the plan that’s right for you at a dollar figure that makes sense for your budget.Īlso worth pointing out, there are a few services on the market - like we saw in our hands-on review of Experian IdentityWorks - that let you “try it before you buy it.” The trial periods we’ve seen usually run between 7 to 30 days, and are really helpful in determining if a particular service is right for you. Basic plans are obviously less expensive and will offer fewer features while premium plans will cost more while doing more. ![]() In our experience, we’ve found that most services on the market offer different tiers of protection. With that said, just because something is expensive doesn’t always mean it’s better - what’s really important is finding identity theft coverage with the features you want while not paying for superfluous functionality. ![]() Bare-bones services like we saw when we reviewed Zander will run around $7 per month, while fully decked-out products like we found in our NortonLifeLock review can run up to around $30 monthly. One of the most important factors when selecting an identity theft protection plan is the cost, but true to the saying - you get what you pay for. Pricing Breakdown: What Will Identity Protection Cost? ![]() ![]() ![]() Other company and product names used herein may be trademarks of their respective owners and are used for the benefit of those owners. Aragami: Nightfall is a new campaign taking place. 2 The players take the role of Aragami, an assassin with supernatural abilities. 1 The game was originally titled Twin Souls: The Path of Shadows. All Games PC PS5 PS4 Xbox Series X Xbox One Switch Wii U 3DS PS3 Xbox 360 PS Vita. Calendar Most Anticipated My Calendar Games. or its licensors and may be registered in some countries. Aragami: Nightfall is the first story expansion to the celebrated stealth-action game by Lince Works. Aragami () is an action-adventure stealth video game developed and published by Lince Works for Linux, Microsoft Windows, OS X, PlayStation 4, Xbox One and Nintendo Switch. Get Aragami - Nightfall PlayStation 4 and PC Release Date, Trailers, News & Reviews. The Lince Works and Aragami logos and all other trademarks used herein are owned by Lince Works S.L. Reviews Aragami is a superb stealth game with a gorgeous visual design and a. Play the Aragami: Nightfall story campaign with a friend in cross-platform online multiplayer. Aragami: Nightfall 9.99 19.97 Add all DLC to Cart.Play 4 new story chapters through winter valleys and decayed cities to stop a grim ritual.Try 3 new Shadow Techniques to help you fulfill your mission.Play as Hyo or Shinobu in Aragami: Nightfall and uncover the fate of the last Shadow Assassins.Play as Hyo and Shinobu and use new Shadow Powers in a story campaign that will put your stealth skills to test. In Aragami: Nightfall, shadow assassins Hyo and Shinobu go after the mysterious ‘Alchemist’ in one last mission to bring back a long lost companion. Try 3 new Shadow Techniques to help you fulfill your mission. Aragami: Nightfall is a new campaign taking place before the events of Aragami, which will provide you with new challenges and more complex scenarios for experienced players, as well as proving a crucial chapter in the story of Aragami, in which you will play the events that led to the awakening of Aragami. Play as Hyo or Shinobu in Aragami: Nightfall and uncover the fate of the last Shadow Assassins. Aragami: Nightfall is a worthy expansion for the stealth game developed by Lince Works, with four new story chapters taking place before the awakening of. Aragami Nightfall is a new campaign taking place before. It told the story of Aragami, the titular supernatural assassin who used darkness to navigate the game’s world and to take out the Kaiho, an army of warriors. Aragami Nightfall is the first story expansion to the celebrated stealth-action game by Lince Works. Play the Aragami: Nightfall story campaign with a friend in cross-platform online multiplayer.Aragami: Nightfall is the first story expansion to the celebrated stealth-action game by Lince Works. 2016’s Aragami was a third-person stealth action game that was reasonably well received and caught our interest because it looked like the Tenchu game we’ve always wanted on the PS4. Play 4 new story chapters through winter valleys and decayed cities to stop a grim ritual. Yet here we are with a brand new expansion for the game. Try 3 new Shadow Techniques to help you fulfill your mission. Its been a long time since Aragami was on anyones mind, having released way back in October 2016. NOTE: Returns and refunds are not possible for this product. Play as Hyo or Shinobu in Aragami: Nightfall and uncover the fate of the last Shadow Assassins. This product is valid for 10 years from the date of purchase and is free of service charges. Jin PS4 tagged aragami / dlc / nightfall / ninja / stealth by Richie 2016’s Aragami was a third-person stealth action game that was reasonably well received and caught our interest because it looked like the Tenchu game we’ve always wanted on the PS4. In Aragami: Nightfall, shadow assassins Hyo and Shinobu go after the mysterious ‘Alchemist' in one last mission to bring back a long lost companion. Aragami: Nightfall is a new campaign taking place before the events of Aragami, which will provide you with new challenges and more complex scenarios for experienced players, as well as proving a crucial chapter in the story of Aragami, in which you will play the events that led to the awakening of Aragami. Aragami: Nightfall is the first story expansion to the celebrated stealth-action game by Lince Works. ![]() ![]() ![]() The Gap killer will make sure there is no silence between tracks - providing uninterrupted music all the time. ![]() Drag & Drop your tracks where you need them multiple categories, or make use of one of the ‘Auto generated’ categories that instantly sorts your songs into various groups. Our smart crossfader will automatically detect the very best point to start fading tracks, or you can manually specify the best point. SAM DJ delivers professional sound with cross-fading, gap killer, beat matching, volume normalization and 5-band compressor/limiter. The cue channel allows you to preview the songs over headphones before you play it live to the audience. Also allows for professional sounding cross-fading between tracks. Latest Version: SAM Broadcaster PRO 2023. Media Library: Organize large music libraries quickly into multiple categories, import information from tags and automatically look up track information via .ĭual deck players: Dual Deck players allow you to queue and position the next song while another song is playing - or let Auto DJ take care of it for you. Access data logs for performance reporting and other useful reports on your station playback history. Listener Statistics and reporting: See in real-time how your audience is growing. Keep your listeners engaged, show them now playing info, let them browse your media library and even request their favorite song! Start your Internet radio station with SAM Broadcaster, the number one Internet Broadcasting Solution since 2003 Sound Professional: Advanced audio features like cross-fade detection, gap killer, volume normalization and 5-band compressor and limiter. ![]() Web Integration: Give your station website a pro look with our easy to integrate web widgets. Atlanta: Joey Loganos full circle win and split personality the spotters are the story Denny Hamlins fine and appeal, Josh Williams controversy. Servers include SAM Broadcaster Cloud,, , Shoutcast v1, Shoutcast v2, Icecast and Windows Media. Stream in multiple formats: Formats include AAC, aacPlus, AAC-HE, MP3, mp3PRO, Ogg and Windows Media. I think the suits in charge know this full well and rely on our love of the sport and lack of alternative access to it to. Sound Professional Advanced audio features like cross-fade detection, gap killer, volume. Sound Professional: Advanced audio features like cross-fade detection, gap killer, volume normalization and 5-band compressor and limiter. SAM Broadcaster takes care of all the major aspects of running a successful internet radio station. Start your Internet radio station with SAM Broadcaster, the number one Internet Broadcasting Solution since 2003 ![]() ![]() ![]() ![]() Insights into the molecular events underlying internal entry of ribosomesĪ chimeric protein formed from a domain of the translation initiation factor eIF4G and the iron responsive element binding protein-1 (IRP-1) is sufficient to promote translation of the downstream cistron of a bicistronic mRNA bearing the iron responsive element (IRE) RNA sequence in the intercistronic space ( De Gregorio et al., 1999). Since then, and particularly over the last three years, IRES activities have been detected in a restricted but increasing number of cellular mRNAs from yeast, Drosophila, birds and mammals, showing that the internal ribosome entry process is far more extensive than previously thought (see Supplementary data). Indeed, the first cellular IRES was identified in the 220-nucleotide-long 5′ UTR of the immunoglobulin heavy chain-binding protein (BiP) mRNA, whose translation is maintained in poliovirus-infected cells at a time when cap-dependent translation is severely inhibited ( Macejak and Sarnow, 1991). These discoveries argued for an alternative mechanism such as the internal entry of ribosomes. Moreover, a few cellular mRNAs are translated preferentially when cap-dependent initiation of translation is impaired. Their presence in viruses as diverse as flaviviruses, retroviruses and even DNA viruses such as the Kaposi’s sarcoma-associated herpesvirus reveals the widespread nature of these RNA elements (see Supplementary data, available at EMBO reports Online).Īs is the case for many viral mRNAs, a number of cellular mRNAs possess structural features in their 5′ UTRs that make them unlikely to be translated by a 5′ cap-dependent ribosome-scanning mechanism. Using this assay as the basis for defining IRESs, these elements have been found in all picornavirus genera. This strategy can be considered the ‘gold standard’ for characterizing IRESs ( Sachs, 2000) if one considers the presence of cryptic RNA processing signals or promoter sequences in the intercistronic space as having been ruled out ( Kozak, 2001). This was first shown for poliovirus, where inserting a segment of the 5′ UTR of a poliovirus genome between the two ORFs allows translation of the downstream cistron, independent of the cap-mediated translation of the first cistron. Bicistronic RNAs with two non-overlapping open reading frames (ORFs) were shown to be good models to test cap-independent translation initiation. The poliovirus and encephalomyocarditis virus (EMCV) 5′ UTRs were the first to be described to ‘break the rule’ of translation initiation ( Jackson, 1988 Jang et al., 1988 Pelletier and Sonenberg, 1988). Nevertheless, these 5′ UTRs confer efficient 40S joining. Their 5′ UTRs also have complex features predicted to impair ribosome recruitment and linear scanning: (i) a long leader sequence (ii) stable secondary structures and (iii) potential upstream initiation codons. Unlike their cellular counterparts, picornaviral mRNAs are naturally uncapped at their 5′ end. Studies on viral gene translation were essential for the initial discovery of internal entry of ribosomes. How widespread is internal ribosome entry? ![]() (An IRES database website is available at. This process is called internal entry of ribosomes and is mediated by internal sequences called internal ribosome entry sites (IRESs). In addition, studies on the translation of viral and eukaryotic mRNAs bearing uncommon features in their 5′ untranslated regions (UTRs) have shed light on an alternative mode of 40S recruitment to the mRNA. The conventional mode of attachment of the 40S ribosomal subunit to the mRNA requires the eIF4F protein adaptor complex, which bridges the 7methyl guanosine capped structure located at the 5′ end of the mRNA and the 40S subunit carrying the eIF3 and eIF2-GTP–Met-tRNA complexes (see Figure 1). In eukaryotes, the situation has proved to be more complicated. This RNA–RNA interaction brings the prokaryotic 30S subunit to an internal position on the mRNA, in the vicinity of an AUG initiation codon. In prokaryotes, the process of ribosome recruitment to the mRNA results from direct base-pairing between the 18S ribosomal RNA and the mRNA Shine–Dalgarno sequence. ![]() |
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